Functional Analysis of Glucose-6-Phosphate Translocator PoGPT1 in Embryogenic Callus Formation of Tree Peony

Tree peony (Paeonia ostii) is an ornamental flowering plant that generally recalcitrant to establishment of a mature somatic embryo regeneration system in vitro. Glucose-6-phosphate translocator (GPT) plays important regulatory role embryogenesis plants. In this study, PoGPT1 was cloned, and bioinformatic analysis functional verification the gene were performed. The results showed encoded polypeptide 392 amino acids, which basic non-secreted hydrophobic transmembrane protein, mainly localized plastids. highly expressed tree leaves, its transcript abundance increased with progression zygotic development. Overexpression caused up-regulation leafy cotyledon 1 (PoLEC1), receptor-like kinase (PoSERK), agamous-like15 (PoAGL15) callus. addition, overexpression promoted increase indole-3-acetic acid (IAA), 5-deoxystrigol (5DS), brassinolide (BL) contents, especially IAA, but reduced contents abscisic (ABA), 6-benzyladenosine (BARP), 1-aminocyclopropanecarboxylic (ACC). present research synergistically regulated endogenous hormones expression levels embryogenesis-related genes promote embryonic development peony. provide theoretical technical support for embryogenic callus formation subsequent on embryogenesis.